Insertion junction: CLIP2.033859_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.033859
Chromosome:chromosome 1
Location:1734302
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g009575 (1 of 11) IPR001471//IPR016177//IPR031112 - AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTGAGAAGTGGATCAAGTCCGGCTTCGGCGCCTTCCCACACCTCAGCG
Internal bar code:GTGCTGGACATGCATTTGACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1841
LEAP-Seq percent confirming:64.0
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:AATGACGGGTCAGGTCAACC
Suggested primer 2:GCTGTTCGCTCTGTCCTTCT