Insertion junction: CLIP2.033860_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.033860
Chromosome:chromosome 4
Location:2260861
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g220500 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGTACCCGCAGCCCATCGCGCAGCACATGACGGTCGTCACGTGCGAGC
Internal bar code:AGATTACGATGAAACTGGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:2985
LEAP-Seq percent confirming:94.4444
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGTGCTCACCACCTTGTA
Suggested primer 2:CCTGGACACCTTCGACTTCC