| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.033862 |
| Chromosome: | chromosome 9 |
| Location: | 171915 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386735 | DLA1 | Dihydrolipoamide acetyltransferase; (1 of 1) PTHR23151//PTHR23151:SF9 - DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED // DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACAAACACCAACCTGAGTCATCGTGGGCGACAGCGACGGCATGTTGAG |
| Internal bar code: | AGGCCTTACGATGGAGCTGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5684 |
| LEAP-Seq percent confirming: | 79.1045 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCCATAGCACGAATAGGT |
| Suggested primer 2: | GTACGGTATGCAGGGTCGAG |