| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.033862 |
| Chromosome: | chromosome 9 |
| Location: | 171927 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386735 | DLA1 | Dihydrolipoamide acetyltransferase; (1 of 1) PTHR23151//PTHR23151:SF9 - DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED // DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGTTTGTTGGTAGTTGGGATCAGCTGACTATTGCACCGGGGGAGGCT |
| Internal bar code: | CGGAGTATGGCGGTGAGAGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 960 |
| LEAP-Seq percent confirming: | 61.2245 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGGTATGCAGGGTCGAG |
| Suggested primer 2: | CCGCCATAGCACGAATAGGT |