Insertion junction: CLIP2.033878_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.033878
Chromosome:chromosome 9
Location:3217120
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394658 LCI28 (1 of 1) 1.1.1.21 - Aldehyde reductase / Polyol dehydrogenase (NADP(+)); Low-CO2-induced aldose reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGGCACCATACACGATAATCAGGTGCTTCACACCTTTGACGTGATGAA
Internal bar code:TTTCCATTACTGGCATGTACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2209
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAGAATGAGCACGAGGTG
Suggested primer 2:TCCAGATAGGACACCTGGGG