Insertion junction: CLIP2.033881_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.033881
Chromosome:chromosome 3
Location:7721675
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g203550 GT90-3,GT90F3 GT90 family protein 3; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAACCCCAACCCCCCCCCCCCACATACACACGCACACGCACGCAACTG
Internal bar code:GTGGTTTAAAGTGGTTTAAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:462
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGTGCGTGTATGTGTGT
Suggested primer 2:TCTTCTGCATTGGGTTCGCT