Insertion junction: CLIP2.033886_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.033886
Chromosome:chromosome 7
Location:2925305
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g332700 (1 of 1) PF00069//PF13639 - Protein kinase domain (Pkinase) // Ring finger domain (zf-RING_2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCGGGAAGGAGGGCCGCATCAAGCATGCCCGGCAGAACGTGTGCCCGC
Internal bar code:AAAGGACATAATATTACGGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2921
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTGCTGTACTGGTGGTCT
Suggested primer 2:TTGAGGTCAGCCATCACCAC