| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033908 |
| Chromosome: | chromosome 17 |
| Location: | 1288605 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705300 | PHC68 | Putative pherophorin-chlamydomonas homolog; (1 of 6) 3.2.1.4 - Cellulase / Endoglucanase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACTGCTGCGGCGTTAGTAACCGTGCTTGCTCCCACGGTGGACCTTCC |
| Internal bar code: | ATTTCGTCGATCCGACCTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3874 |
| LEAP-Seq percent confirming: | 94.8276 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTCGTTTTCTCTCGCCA |
| Suggested primer 2: | AGCGTAGGGTAGGGGTTGAT |