| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033937 |
| Chromosome: | chromosome 12 |
| Location: | 458563 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801344 | (1 of 5) PTHR19446:SF107 - INTRON MATURASE, TYPE II FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGCCAAGAATGCAGCCATCATGGAAAGTTGGATTCGGCTGCAGGGTA |
| Internal bar code: | AATCTAGGCTGCTAAGGAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1183 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTCGGGTCTTTAGCTGGC |
| Suggested primer 2: | GAGGGCTAGCGCTAAGACAG |