Insertion junction: CLIP2.033947_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.033947
Chromosome:chromosome 6
Location:381283
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g251550 SPP3,SPPA1-3,SPP1C Signal peptide peptidase; (1 of 1) IPR002142//IPR029045 - Peptidase S49 // ClpP/crotonase-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGGTGGACGCTGTTGATCCTAGCCCGCTCAGGGGCCCTACAACCCAGG
Internal bar code:AGCTTTGTGTAACACCATGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:842
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGCAGCTCAATCTTCAGG
Suggested primer 2:TCCCTGTTGTTCGTCTCAGC