Insertion junction: CLIP2.033950_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.033950
Chromosome:chromosome 7
Location:5994691
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g354600 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCGCCGCCTCGCCCACCGCCGCCTTCGCCGCGGCCAGCCAGGAGCAGG
Internal bar code:TGGTAGTTGTTTTCGTTACTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:157
LEAP-Seq percent confirming:15.7895
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TCCAAAACCTTTGCCCCAGT
Suggested primer 2:TGTGTGGGCCAAAGTCTTGT