| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.033961 |
| Chromosome: | chromosome 12 |
| Location: | 4978800 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524900 | (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGCATGGCGACAAATGGTGGTGACCCCTGACTGGGCGACTATCGCAA |
| Internal bar code: | TCCTCACATACGGGTATCCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2015 |
| LEAP-Seq percent confirming: | 46.6667 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGACGGAGCTGCAAGTGA |
| Suggested primer 2: | TTTACACGCCTGGGATTGCT |