Insertion junction: CLIP2.033981_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.033981
Chromosome:chromosome 12
Location:4989471
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g525050 SMC6A (1 of 2) PTHR19306//PTHR19306:SF6 - STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6 // PROTEIN C23H4.6, ISOFORM A-RELATED; Structural Maintenance of Chromosomes protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGTGCAGTAGCATCAGACTCGCCCACGCAAACAGCAAACCCCCCCATG
Internal bar code:GGGAACCTAATTATGGCATGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:7676
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:GGATCAAGCGCGACCATTTC
Suggested primer 2:CTCTTTCACGCGCTCATTCG