Insertion junction: CLIP2.033983_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.033983
Chromosome:chromosome 2
Location:6952102
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g389208 (1 of 2) PTHR11266//PTHR11266:SF17 - PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17 // PROTEIN MPV17 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAAGTGGATGCACGCCGTGTGGTCACTACAGCAGCATTTGGCGCGTGC
Internal bar code:CGGCTTGTCCAATCATCCCCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3972
LEAP-Seq percent confirming:98.6487
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:GCCAGTATACGACGTCCTGG
Suggested primer 2:CTCTTGCATCCTTTGGGGGT