| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033991 |
| Chromosome: | chromosome 5 |
| Location: | 1217562 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241633 | (1 of 2) IPR006626//IPR011050//IPR012334 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor // Pectin lyase fold | intron | |
| lncRNA_TCONS_00245550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGGGCCGGAAACCAGTGCCGCGTTCTGTGCTGCACCCAGCTGCATT |
| Internal bar code: | CTTTATTCGTTATTGTGAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1142 |
| LEAP-Seq percent confirming: | 53.1915 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTCGTTTTCGTTGGTTT |
| Suggested primer 2: | ATGCGGTCTGGTGAGGAATG |