Insertion junction: CLIP2.034005_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.034005
Chromosome:chromosome 2
Location:2195985
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g089608 (1 of 1) K11136 - regulator of telomere elongation helicase 1 [EC:3.6.4.12] (RTEL1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGGCCGAACGGCAGCACACCCACCCACACCTGGACAGGGGCAGCAGCA
Internal bar code:CCCGCGCAAATAGACCACATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:411
LEAP-Seq percent confirming:95.8333
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCTCCCCTTTCGGATGAT
Suggested primer 2:GAGGTGGGGAGAAGGGAGAT