Insertion junction: CLIP2.034005_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.034005
Chromosome:chromosome 2
Location:2195986
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g089608 (1 of 1) K11136 - regulator of telomere elongation helicase 1 [EC:3.6.4.12] (RTEL1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCGGCACCCCCCTCAACTCCACATACGCCTCGCGTGACAGCGTGGCTTA
Internal bar code:CCCGCGCAAATAGACCACATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:612
LEAP-Seq percent confirming:10.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGTGGGGAGAAGGGAGAT
Suggested primer 2:TCCCTCCCCTTTCGGATGAT