| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.034006 |
| Chromosome: | chromosome 6 |
| Location: | 1190902 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258300 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGAACCAATCCGTGTGTGTGTGCGTGTGCACATGTGTGTGACCAGGCG |
| Internal bar code: | ATAACGGGTAAGATACTGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3547 |
| LEAP-Seq percent confirming: | 85.2941 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGATCTAGCCGAGGAACC |
| Suggested primer 2: | TGCATAGGAGGCGATCGAAC |