Insertion junction: CLIP2.034045_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.034045
Chromosome:chromosome 10
Location:1284169
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g426900 (1 of 3) PF02458 - Transferase family (Transferase) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCGGCGCCTTTGATGTGTCCTGCTGCAACAGCGGCGCCCGGCTGGTGA
Internal bar code:GCCTAGGGGTTGGGGTCTCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2063
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:87

Suggested primers for confirmation by PCR

Suggested primer 1:GCACCTTGTCCTGCACAATG
Suggested primer 2:TGAGTGACGTGTGTGCTCAG