| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.034047 |
| Chromosome: | chromosome 6 |
| Location: | 1856282 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263800 | SEE2 | Putative splicing endonuclease positive effector; (1 of 1) PTHR10887:SF369 - DNA2/NAM7 HELICASE FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCTGCTGTCACCTCTGGGCCCATAAGAACCAGCAGCTGCCTTGGCCC |
| Internal bar code: | AGATGGTTGGTTGCAAGCTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTACTGTAGCTTAGCGGG |
| Suggested primer 2: | GTAACCACACACGCACACAC |