| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.034056 |
| Chromosome: | chromosome 12 |
| Location: | 4365498 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801409 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTTGCGCTGTCACTTCGGGAAAGTATGGGGATGGCTATGGGTTTCCT |
| Internal bar code: | TACTTGTACAGGTACTAAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3472 |
| LEAP-Seq percent confirming: | 89.2857 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTATCAGGGCCCAACTTG |
| Suggested primer 2: | CCATCCTGCTCACTGCCTAC |