Insertion junction: CLIP2.034067_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.034067
Chromosome:chromosome 6
Location:6453203
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g293800 ARS16 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGATCCCAGACACCCGCATCGACATGACCAAGTGCGTGAGGGCGTGGG
Internal bar code:GTAGTCGAGTGATCGCAGTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1750
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAATCCTTCCTGTCCCCC
Suggested primer 2:GTACATAGAGTGCGTCCGGG