| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.034077 |
| Chromosome: | chromosome 12 |
| Location: | 8236811 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551600 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGAGCCGAGCACACACGAGACTGCTGCTCGTACTTGATCTGCTTGCT |
| Internal bar code: | CTGGGGCACAAAGCAGGACGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3211 |
| LEAP-Seq percent confirming: | 86.9048 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAAGCGAAACGGAGATGA |
| Suggested primer 2: | CAAGCGCACATACGTCTTCG |