| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.034086 |
| Chromosome: | chromosome 10 |
| Location: | 4710824 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452300 | (1 of 4) 2.1.1.62 - mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACACGCACAAACATGGCGGGAGCGCAGGGTAGAGGGGCGACCACCA |
| Internal bar code: | GCTTATTAGTAATTGGTAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1715 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTTCTAGACAGCCAGCG |
| Suggested primer 2: | CTAGACAGCGTCCATCGTCC |