| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.034095 |
| Chromosome: | chromosome 7 |
| Location: | 998834 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319500 | (1 of 2) 2.3.2.15 - Glutathione gamma-glutamylcysteinyltransferase / Phytochelatin synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGACAGGCTGACACCAACCCAAGCCCGATTCCCTGTCCGTGCCGAAGCA |
| Internal bar code: | GATGAGCGAGGTATGAGCGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1572 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAACCACTCCGCTCCACAC |
| Suggested primer 2: | CAAGAACGGAGGCCAAGACT |