Insertion junction: CLIP2.034105_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.034105
Chromosome:chromosome 4
Location:2103391
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g219700 BBS9 (1 of 1) K19398 - Bardet-Biedl syndrome 9 protein (BBS9); Bardet-Biedl syndrome-9 associated protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAAACCCTTCAAAGTCACACACCTCCCCCCCACACCCCGCCTGGATGC
Internal bar code:TCCATCTTTATTTCTCTCATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:220
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCTCGTTTTCGTTGGTTT
Suggested primer 2:TGAGCGCAAACAAGGAAACG