Insertion junction: CLIP2.034106_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.034106
Chromosome:chromosome 6
Location:6177499
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g291950 MRS4,MRS3 Mg2+ transporter protein; (1 of 3) K16075 - magnesium transporter (MRS2, MFM1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCAGCACACAGTCCGCCACACGCCCACCCAACCAACCGCCGCCGCTGC
Internal bar code:ATGTTTGCTGTGTGCCGAGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:486
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGATGATCATCGGGGCAG
Suggested primer 2:AGAGGGTGCAAAGACTGGTG