Insertion junction: CLIP2.034119_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.034119
Chromosome:chromosome 14
Location:1858911
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g619850 ELG36 (1 of 2) 2.7.7.52 - RNA uridylyltransferase / TUT; Exostosin-like membrane protein, a heparan sulfate glycosyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCGTCACGTTGAAGCATCACGCGTGGTGCGAAGGCGTCTTTAGCTTGT
Internal bar code:CTGGATCCCTGCGAGGTCGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1231
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAGTCACGAGCTTTGGAG
Suggested primer 2:AAACAACAACGGCGATGTGG