| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.034125 |
| Chromosome: | chromosome 12 |
| Location: | 8268868 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551503 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTGCCACTCGGGGTCAGGTCGTTCGTCGATCGCCCGCGGGGGGGTTC |
| Internal bar code: | AATTCGAGAAAATTTTATTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 10.7143 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATGTCGCTTCCTTCACC |
| Suggested primer 2: | TGCTTTCTAGTGCTCCGCAA |