| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.034129 |
| Chromosome: | chromosome 1 |
| Location: | 2805865 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g017250 | FKB99 | Chlorophyceae-specific protein; (1 of 2) K09571 - FK506-binding protein 4/5 (FKBP4_5) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGCCGCGGCCCGGCCCGGACCGCCCGCCCGCCGCCACCGCCGCGCCG |
| Internal bar code: | GATCTAGGCTATAAGTCGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 971 |
| LEAP-Seq percent confirming: | 4.08163 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACACCAGCAACCTGTCGT |
| Suggested primer 2: | GCAGGCTCCATTCAAGTCCT |