| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034144 |
| Chromosome: | chromosome 2 |
| Location: | 5379928 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800236 | (1 of 2) 6.1.1.21 - Histidine--tRNA ligase / Histidyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTTGACCGTTGACACGGTGCGCGTGTCAACAGCCGCAAATGCCACTT |
| Internal bar code: | TGGGGCGTTGCCTCATTTAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 104 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCCCTCCCCTCCTTAGC |
| Suggested primer 2: | ATACACACGGGCACACTGAG |