| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034176 |
| Chromosome: | chromosome 6 |
| Location: | 4962539 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGTTATGCCACCAAGCAAGGAACGTAACTAGCTACTTGGCCTGGTCT |
| Internal bar code: | TGGTTTTCGGTTATCCAATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 69.697 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATCTTGTTGAGCAGGCCC |
| Suggested primer 2: | AACCCAGGTAGCAGTTTCGG |