| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034177 |
| Chromosome: | chromosome 6 |
| Location: | 33759 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g248800 | (1 of 1) PTHR31109:SF2 - PROTEIN FAM207A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTGTCAATCAGGGACCTCCAGGCGGGCGGGGGTGGCAAGCCATAGGC |
| Internal bar code: | ATTAGGGCATTGTACGGTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6108 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTTAGATGTTCGCAGCG |
| Suggested primer 2: | GGGCGCGACATATCTACACA |