| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.034179 |
| Chromosome: | chromosome 7 |
| Location: | 2675554 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330800 | OPR30 | OctotricoPeptide Repeat protein 30 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGCTCCCACCTGCAGCGCCAGCGGCCACACCGCCGCCAGCTCCGGCC |
| Internal bar code: | ATTGTACACGTCTGATTATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1632 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATCCATGGACCTGCAGAG |
| Suggested primer 2: | CCAGGTACAGCTGTGACAGG |