| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.034180 |
| Chromosome: | chromosome 2 |
| Location: | 6704777 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387319 | (1 of 2) PTHR11082//PTHR11082:SF25 - TRNA-DIHYDROURIDINE SYNTHASE // TRNA-DIHYDROURIDINE SYNTHASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGCGCGTGGCGGCGGCTCAGCGCTGCCGCTGCGGCCGCGCCTGTTTG |
| Internal bar code: | CTCACATGATGTTCGTTACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1918 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCGGAACTTGACGGTCA |
| Suggested primer 2: | GACTCCTGCACTGTCAACCA |