Insertion junction: CLIP2.034185_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.034185
Chromosome:chromosome 7
Location:3323954
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGACGGGATGAGGGGCACGGCGGTGGCGCTGGTAAGTCACCAACACGC
Internal bar code:TTGAGTTCGACCAGGCGTGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2583
LEAP-Seq percent confirming:96.6102
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCTATCCCACACCCGACA
Suggested primer 2:GCAAACCCGTTCTGACACAC