Insertion junction: CLIP2.034216_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.034216
Chromosome:chromosome 1
Location:5979499
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g042450 (1 of 14) IPR006626//IPR011050 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCATCTTTCCAGGGGCCCGCCTGTTCCACGCAGGGTTCAGTAGAATCG
Internal bar code:CTGGTGAGGATTACCGTGTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2143
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTGTCCTGCATCGTGTGA
Suggested primer 2:ATGTGGCTCCGTTGGGATTT