| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.034234 |
| Chromosome: | chromosome 2 |
| Location: | 398971 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075750 | (1 of 13) PF00646 - F-box domain (F-box) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGAACCTGGTCTTGCACACCCACCCACCCACACACACACACACACAC |
| Internal bar code: | GTGCGAAACGCATAGTTATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1392 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTGTTGCTGTTGCTAC |
| Suggested primer 2: | TGCGGCTTATTGACCCCTTT |