Insertion junction: CLIP2.034242_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.034242
Chromosome:chromosome 12
Location:1630480
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g486850 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTCCATGTGGACGGACGGGCCATTGGTATACGAATTCTGGGCAAGTGG
Internal bar code:GTGTCTAATTACGACCCGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2502
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:AGCACATGCATCCCACATCA
Suggested primer 2:CATGGAACTCGGTCACACCA