| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.034248 |
| Chromosome: | chromosome 7 |
| Location: | 4934980 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346750 | PUS14 | (1 of 2) 5.4.99.28//5.4.99.29 - tRNA pseudouridine(32) synthase / Pseudouridine synthase RluA // 23S rRNA pseudouridine(746) synthase / 23S RNA Psi(746) synthase; RNA pseudouridine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGCGGCGGGTGTCAGGTGGTGACCGGGCCGACTCACCGGTCAGACTG |
| Internal bar code: | AAATTTTCGTTAGTCCTTTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2069 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGCCCTGAAGGTGAACC |
| Suggested primer 2: | TCCGTCAATACACACACGCA |