| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.034262 |
| Chromosome: | chromosome 7 |
| Location: | 4728896 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g345400 | FAP70 | Flagellar Associated Protein 70; (1 of 1) PTHR23083//PTHR23083:SF418 - TETRATRICOPEPTIDE REPEAT PROTEIN, TPR // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACGACACTGGCCCTCCGTGGGACCCGACACACGCACACTACACGCGCT |
| Internal bar code: | ACTATGATTACTATTGCGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGACTTGCGTTGTCGTGC |
| Suggested primer 2: | CAGAACACTCACTCGCACCT |