| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.034279 |
| Chromosome: | chromosome 1 |
| Location: | 4177996 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028000 | POB12 | Proteome of basal body 12; (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAGCAGGATCCTCGACGCTCCATCACAAAGGCTGCTGCAGGGCCACCC |
| Internal bar code: | ATTGCCCGTAGCTATGTCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2383 |
| LEAP-Seq percent confirming: | 98.75 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCCTTCAATGTGTTTCGC |
| Suggested primer 2: | TGCCCATACTCAAACGCCAT |