| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.034287 |
| Chromosome: | chromosome 16 |
| Location: | 1576940 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653700 | (1 of 1) PTHR10972//PTHR10972:SF71 - OXYSTEROL-BINDING PROTEIN-RELATED // OXYSTEROL-BINDING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGAGTGCGCGTGCGGGGTGGCTCGGCGAGTGACCTCACGCCAGGCCAG |
| Internal bar code: | GATGGTCGGATGGGGGTGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4254 |
| LEAP-Seq percent confirming: | 78.125 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGTGGTTGCGCTCTTGC |
| Suggested primer 2: | AAAAGCATAGGCCACGCTCT |