| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.034291 |
| Chromosome: | chromosome 12 |
| Location: | 4766921 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523150 | PFD5 | Prefoldin alpha subunit; (1 of 1) K04797 - prefoldin alpha subunit (pfdA, PFDN5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATGACTTGATGGGGTGTGGGGCGGGTCAGCTTGGCATGTGCCTCCAGT |
| Internal bar code: | TAGTCTTCTATACGAGTGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 987 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAACCACTGGCTTGCAAC |
| Suggested primer 2: | AGAAGTCCGTGGAGCAACTG |