| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034307 |
| Chromosome: | chromosome 16 |
| Location: | 6374701 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675600 | (1 of 5) IPR000104//IPR001214 - Antifreeze protein, type I // SET domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTAGGTCGACACCGGCAGGACCGTGGGCATGCCGTGGCGGCGCGTCG |
| Internal bar code: | CCCGGAGTTCGGGGCTTTGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4066 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACACCGATATAGACGCA |
| Suggested primer 2: | CTTTCCAGCGCTTCCTCCAT |