| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.034309 |
| Chromosome: | chromosome 11 |
| Location: | 3630433 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478184 | (1 of 1) 3.2.1.25 - Beta-mannosidase / Mannase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAACTGGAGGGCGGCGAAGTGTAAAAGGCAGCAGGTGGCAGGAGAGCGT |
| Internal bar code: | CATATATTGATGCCCCGAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 400 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAGCTCGCTGGACTATG |
| Suggested primer 2: | CCATCCTCCTCCTCCTTCCA |