| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.034336 |
| Chromosome: | chromosome 3 |
| Location: | 5375983 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183451 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATGTGCAGCGGTGGCTGCAGCAGGCCCTGCGGCGGTAACCCCTGGGC |
| Internal bar code: | TACAGGTTAGGGACGATAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 237 |
| LEAP-Seq percent confirming: | 21.7391 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACAGCTCCCACGACACAC |
| Suggested primer 2: | TGCACCTCCGATGAAAGGTC |