Insertion junction: CLIP2.034394_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.034394
Chromosome:chromosome 4
Location:2558286
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g222550 (1 of 43) IPR011989 - Armadillo-like helical CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATCCAGACGCAGTGGCGGACGCTGAGATGTACGGCGGTGGCGGTGGCG
Internal bar code:TTTAGTCAAGGGAACAGATAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1789
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCCCTCCTCTCCATCATA
Suggested primer 2:CACACACACACACACACACC