| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034409 |
| Chromosome: | chromosome 3 |
| Location: | 6504138 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193850 | SUCLG1,SCLA1,TEF25,SCL1 | (1 of 1) K01899 - succinyl-CoA synthetase alpha subunit (LSC1); Succinyl-CoA ligase alpha chain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCCAGGGCCTGACCGGCAAGAATGGAACCTTTCACACGGAACAGGTG |
| Internal bar code: | GGACGGGAGTAAGGGCCCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2936 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCAACAGCCTCCAGAAT |
| Suggested primer 2: | AATGGGGACTTCATGCCTGG |