Insertion junction: CLIP2.034438_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.034438
Chromosome:chromosome 1
Location:4929168
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g034200 (1 of 7) PF00211//PF13416 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Bacterial extracellular solute-binding protein (SBP_bac_8) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCAGCTAGCCTCACACCAACCTCTGCCCGCCGCGCCACGTCAATGTGG
Internal bar code:CACATGGCTAATGGCGCTATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2006
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:GACGATGTTTGGGCTCTCCA
Suggested primer 2:CTTCCCCACCTCACAATCCC